Article last updated: October 15, 2024
Human Neonatal Fibroblast/Keratinocyte Conditioned Media, often abbreviated as HNFCM, is a fascinating and somewhat complex ingredient used in the world of cosmetics. This ingredient is derived from the growth media that has been used to culture human neonatal fibroblasts and keratinocytes for several days. These cells are essentially the building blocks of skin, with fibroblasts playing a crucial role in the formation of connective tissue and keratinocytes being the primary type of cell found in the epidermis, the outermost layer of the skin.
The starting media for this process is Minimum Essential Medium Eagle with Earle’s salts, which is a nutrient-rich solution designed to support cell growth. Notably, this medium does not contain L-glutamine and phenol red, and it may or may not include fetal bovine serum, which is often used to provide additional growth factors.
The use of conditioned media in cosmetics is relatively recent but has its roots in the broader field of regenerative medicine and cell biology. Scientists discovered that the media in which these cells are grown becomes enriched with various bioactive compounds, including growth factors, cytokines, and extracellular matrix proteins, which can have beneficial effects on the skin. This discovery led to the incorporation of conditioned media into skincare formulations, aiming to harness these bioactive compounds to improve skin health and appearance.
The process of making Human Neonatal Fibroblast/Keratinocyte Conditioned Media involves culturing the neonatal fibroblasts and keratinocytes in the specified growth medium for a set period. After several days, the media is collected and filtered to remove the cells, leaving behind a solution rich in the secreted bioactive compounds. This conditioned media is then used as an ingredient in various cosmetic products, particularly those targeting skin conditioning and rejuvenation.
In this section, we will delve into the officially recognized cosmetic benefits and uses of Human Neonatal Fibroblast/Keratinocyte Conditioned Media:
Human Neonatal Fibroblast/Keratinocyte Conditioned Media is primarily recognized for its skin conditioning properties. This means it helps to maintain the skin in good condition. In simpler terms, it can make your skin feel softer, smoother, and more hydrated. By supporting the skin’s natural barrier, it can help to improve the overall texture and appearance of your skin, making it look healthier and more radiant.
Note: The listed benefits above are exclusively based on the officially recognized and defined functions of the ingredient, as documented by the International Nomenclature of Cosmetic Ingredients (INCI).
Human Neonatal Fibroblast/Keratinocyte Conditioned Media is generally considered safe for topical application in cosmetic products. However, as with any skincare ingredient, there are potential side effects and considerations to keep in mind.
Regarding individuals who are pregnant or breastfeeding, data and research on the topical usage of Human Neonatal Fibroblast/Keratinocyte Conditioned Media during pregnancy are lacking. Therefore, it is advisable for pregnant or breastfeeding women to consult a healthcare professional before using products containing this ingredient.
Side effects and adverse reactions from this ingredient are generally uncommon. However, it is recommended to perform a patch test before widespread usage to ensure that the product is suitable for your skin.
In terms of comedogenicity, Human Neonatal Fibroblast/Keratinocyte Conditioned Media is rated as a 1 on a scale of 0 to 5, where 0 is totally non-comedogenic and 5 is highly comedogenic. This low rating means it is unlikely to clog pores and is generally suitable for individuals prone to acne, blemishes, or breakouts.
